Ken Berglund, PhD

    Assistant Professor

    Emory University Department of Neurosurgery

    Education

    • BA, University of Tokyo, Japan, 1997
    • MA, University of Tokyo, Japan, 1999
    • PhD, University of Tokyo, Japan, 2002

    Biography

    Honors

    • Travel Award, Japan Neuroscience Society, 2016
    • Postdoctoral Travel Award, Society for Neuroscience, 2013
    • NRSA for Individual Postdoctoral Fellowship, NIH, 2004
    • Postdoctoral Fellowship for Research Abroad, JSPS, 2004 – 2006
    • Postdoctoral Fellowship for Research Abroad, the Uehara Memorial Foundation, 2002
    • Overseas Fellowship, Japan Science and Technology Corporation, 2002 – 2004
    • Research Fellowship, Japan Society for Promotion of Science (JSPS), 2001

    Memberships

    • Society for Neuroscience
    • The Japan Neuroscience Society

    Research

    Development of optogenetic tools and their applications in neuroscience research. Specifically, I have developed and used genetic chloride indicators and bioluminescence-driven channels/pumps to monitor and control, respectively, specific activity of neurons in the rodent brain.

    Publications

    • Naotoshi Minami, Ken Berglund, Takeshi Sakaba, Hiromi Kohmoto, and Masao Tachibana. Potentiation of transmitter release by protein kinase C in goldfish retinal bipolar cells.  Journal of Physiology, October 1998, Vol. 512 (1), pp. 219-225. PMID: 9729631.
    • Henrique von Gersdorff, Takeshi Sakaba, Ken Berglund, and Masao Tachibana. Submillisecond kinetics of glutamate release from a sensory synapse.  Neuron, November 1998, Vol. 21 (5), pp. 1177-1188. PMID: 9856472.
    • Ken Berglund, Mitsuharu Midorikawa, and Masao Tachibana. Increase in the pool size of releasable synaptic vesicles by the activation of protein kinase C in goldfish retinal bipolar cells. Journal of Neuroscience, June 2002, Vol. 22 (12), pp. 4776-4785. PMID: 12077174.
    • Brooks B. Pond, Ken Berglund, Thomas Kuner, Guoping Feng, George J. Augustine, and Rochelle D. Schwartz-Bloom. The chloride transporter NKCC-1 contributes to intracellular chloride increases after in vitro ischemia.  Journal of Neuroscience, February 2006, Vol. 26(5), pp. 1396-1406. PMID: 16452663.
    • Ken Berglund, Wolfram Schleich, Patrik Krieger, Li Shen Loo, Dongqing Wang, Nell B. Cant, Guoping Feng, George J. Augustine, and Thomas Kuner. Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon. Brain Cell Biology, December 2006, Vol. 35(4-6), 207-228. PMID: 18398684.
    • Mitsuharu Midorikawa, Yoshihiko Tsukamoto, Ken Berglund, Masaaki Ishii, and Masao Tachibana. Different roles of ribbon-associated and ribbon-free active zones in retinal bipolar cells. Nature Neuroscience, September 2007, 10(10), pp. 1268-1276. PMID: 17828257.
    • Ken Berglund, Wolfram Schleich, Hong Wang, Guoping Feng, William C. Hall, Thomas Kuner, and George J. Augustine. Imaging synaptic inhibition throughout the brain via genetically targeted Clomeleon. Brain Cell Biology, August 2008, Vol. 36(1-4), 101-118. PMID: 18850274.
    • Michele Yeo, Ken Berglund, George Augustine, and Wolfgang Liedtke. Novel repression of Kcc2 transcription by REST-RE-1 controls developmental switch in neuronal chloride. Journal of Neuroscience, November 2009, Vol. 29(46), 14652-14662. PMID: 19923298.
    • Soojung Lee, Bo-Eun Yoon, Ken Berglund, Soo-Jin Oh, Hyungju Park, Hee-Sup Shin, George J. Augustine, and C. Justin Lee. Channel-mediated tonic GABA release from glia. Science, November 2010, Vol. 330(6005), pp. 790-796. PMID: 20929730.
    • Michele Yeo, Ken Berglund, Michael Hanna, Junjie Guo, Jaya Kittur, Joel Abramowitz, Jorge Busciglio, Yuan Gao, Lutz Birnbaumer, and Wolfgang B. Liedtke. Bisphenol A delays the perinatal chloride shift in cortical neurons by epigenetic effects on the Kcc2/Slc12a5 promoter. PNAS, February 2013, Vol. 110(11), pp. 4315-4320. PMID: 23440186.
    • Ken Berglund, Elisabeth Birkner, George J. Augustine, and Ute Hochgeschwender. Light-emitting channelrhodopsins for combined optogenetic and chemical-genetic control of neurons. PLOS ONE, March 2013, Vol. 8(3), e59759. PMID: 23544095.
    • Wolfgang Liedtke, Michele Yeo, Hongbo Zhang, Yiding Wang, Michelle Gignac, Sara Miller, Ken Berglund, and Jie Liu. Highly conductive carbon nanotube matrix accelerates developmental chloride extrusion in central nervous system neurons by increased expression of chloride transporter KCC2. Small, April 2013, Vol. 9(7), pp. 1066-1075. PMID: 23229576.
    • Brent Asrican, George J. Augustine, Ken Berglund, Susu Chen, Nick Chow, Karl Deisseroth, Guoping Feng, Bernd Gloss, Riichiro Hira, Carolin Hoffman, Haruo Kasai, Malvika Katarya, Jinsook Kim, John Kudolo, Li Ming Lee, Shun Qiang Lo, James Mancuso, Masanori Matsuzaki, Ryuichi Nakajima, Li Qiu, Gregory Tan, Yanxia Tang, Jonathan T. Ting, Sachiko Tsuda, Lei Wen, Xuying Zhang, and Shengli Zhao. Next-generation transgenic mice for optogenetic analysis of neural circuits. Frontiers in Neural Circuits, September 2013, Vol. 7(160). PMID: 24324405.
    • Ken Berglund, Kara Clissold, Haofang E. Li, Lei Wen, Sung Young Park, Jan Gleixner, Marguerita E. Klein, Dongye Lu, Joseph W. Barter, Mark A. Rossi, George J. Augustine, Henry H. Yin, and Ute Hochgeschwender. Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation. PNAS, January 2016, Vol. 113(3), pp. E358- PMID: 26733686.
    • Jack K. Tung, Ken Berglund, Claire-Anne Gutekunst, Ute Hochgeschwender, and Robert E. Gross. Bioluminescence imaging in live cells and animals. Neurophotonics, April 2016, Vol. 3(2).
    • Ken Berglund, Lei Wen, Robert L. Dunbar, Guoping Feng, and George J. Augustine. Optogenetic visualization of presynaptic tonic inhibition of cerebellar parallel fibers. Journal of Neuroscience, May 2016, Vol. 36(21), pp. 5709-5723. PMID: 27225762.
    Review Articles:
    • Ken Berglund and George J. Augustine. Calcium helps neurons identify synaptic targets during development. Neuron, July 2008, 59(2), pp. 186-187. PMID: 18667145.
    • Jack K. Tung, Ken Berglund, and Robert E. Gross. Optogenetic approaches for controlling seizure activity. Brain Stimulation, November-December 2016, Vol. 9(6), pp. 801-810. PMID: 27496002.
    Book Chapters:
    • Ken Berglund, Robert L. Dunbar, Psyche Lee, Guoping Feng, and George J. Augustine. A practical guide: imaging synaptic inhibition with Clomeleon, a genetically encoded chloride indicator. In Imaging in neuroscience and development: A laboratory manual (ed. Rafael Yuste and Arthur Konnerth). pp. 595-598. CSHL Press, Cold Spring Harbor, NY, 2005.
    • Ken Berglund, Thomas Kuner, and George J. Augustine. Clomeleon, a genetically encoded chloride indicator. In Physiology and pathology of chloride transporters and channels in the nervous system (ed. F. Javier Alvarez-Leefmans and Eric Delpire). pp. 125-140. Academic Press, London, UK, 2009.
    • Ken Berglund, Thomas Kuner, Guoping Feng, and George J. Augustine. Imaging synaptic inhibition with the genetically encoded chloride indicator Clomeleon. In Imaging in Neuroscience: A laboratory manual (ed. Fritjof Helmchen and Arthur Konnerth). CSHL Press, Cold Spring Harbor, NY, 2011. PMID: 22135666.
    • Ken Berglund, Lei Wen, and George J. Augustine. Optogenetic sensors for monitoring intracellular chloride. In Optogenetics: Light-sensing proteins and their applications (ed. Hiromu Yawo, Hideki Kandori, and Amane Koizumi). pp. 159-183. Springer Japan, Tokyo, Japan, 2015. doi: 1007/978-4-431-55516-2_11.
    • Ken Berglund, Jack K. Tung, Bryan Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender. Combined optogenetic and chemogenetic control of neurons. In Optogenetics: Methods and protocols (ed. Arash Kianianmomeni). pp. 207-226. Springer, New York, NY, 2016. doi: 1007/978-1-4939-3512-3_14.
    Published Abstracts:
    • Naotoshi Minami, Ken Berglund, Takeshi Sakaba, and Masao Tachibana. Activation of protein kinase C Potentiates transmitter release from goldfish retinal bipolar cells.  Neuroscience Research, 1998, Suppl. 22, p. S77. DOI: 1016/S0168-0102(98)81868-3.
    • Ken Berglund, Naotoshi Minami, and Masao Tachibana. Continuous transmitter release from goldfish retinal bipolar cells.  Neuroscience Research, 1999, Suppl. 23, p. S74. DOI: 1016/S0168-0102(99)00092-9.
    • Ken Berglund, Naotoshi Minami, and Masao Tachibana. Activation of protein kinase C increases the pool size of releasable synaptic vesicles in goldfish retinal bipolar cells. Neuroscience Research, 2000, Suppl. 24, p. S54. DOI: 1016/S0168-0102(00)82943-0.
    • Ken Berglund, Mitsuharu Midorikawa, and Masao Tachibana. Mechanisms of synaptic potentiation by protein kinase C in goldfish retinal bipolar cells.  Japanese Journal of Physiology, 2001, Suppl. 51, p. 190.
    • Ken Berglund, Yoshihiko Tsukamoto, Mitsuharu Midorikawa, and Masao Tachibana. Effects of phorbol ester on the distribution of synaptic vesicles in retinal bipolar cells.  Neuroscience Research, 2001, Suppl. 25, p. S73. DOI: 1016/S0168-0102(01)00312-1.
    • George J. Augustine, Robert Dunbar, Ken Berglund, Lee S. Loo, Guo-Ping Feng, T. Schafer, Wolfram Schleich, and Thomas Kuner. Measuring neuronal chloride signaling with Clomeleon, a ratiometric, genetically encoded indicator. Journal of General Physiology, 2003, Vol. 122(1), p. 2a. DOI: 1085/jgp.20031221abstracts
    • William H. Baldridge, Xu Wang, Marika Forsythe, Ken Berglund, George Augustine, and Luis Pérez de Sevilla Müller. Displaced ganglion cells in the Chlomeleon (CLM1) mouse retina. Investigative Ophthalmology and Visual Science, 2012, Vol. 53(14), p. 1953.
    • Ken Berglund, Maciej Skotak, Fang Wang, Namas Chandra, and Wolfgang Liedtke. Mouse model of mild traumatic brain injury caused by air-blast with ultra-long-term abnormalities in neuronal chloride and social and emotional behavior. Journal of Neurotrauma, August 2013, Vol. 30(15), p. A44. DOI: 1089/neu.2013.9938.
    • Ken Berglund, Kyle A. Matthews, Allen W. Yu, Michele Yeo, Cameron R. Bass, and Wolfgang Liedtke. In vitro model of mild traumatic brain injury (mTBI) caused by air-blast implicates treatable chloride dysregulation. Journal of Neurotrauma, August 2013, Vol. 30(15), p. A175. DOI: 1089/neu.2013.9938.
    Other Publications:
    • Ken Berglund. An insight into the effects of the cultural differences between the United States and Japan on the research environment. Journal of the Physiological Society of Japan, 2004, Vol. 66(7/8), pp. 246 – 247 (in Japanese).
    • Contributed a cover picture for Society for Neuroscience FY2007 Annual Report.